Introduction: Low back pain (LBP) is a significant public health concern, especially among middle-aged and older women. It affects daily activities and leads to disability, impacting quality of life. Low back pain (LBP) is one of the most prevalent musculoskeletal disorders worldwide, affecting individuals of all ages and socioeconomic backgrounds. Among women aged 45-65, LBP is particularly concerning due to physiological, lifestyle, and occupational factors that contribute to its development and persistence. This study aims to assess the prevalence of LBP and its associated disability among women aged 45-65. Materials and Methods: A cross-sectional study was conducted among 200 women aged 45-65. Participants were selected using stratified random sampling. Data were collected using structured questionnaires, including the Oswestry Disability Index (ODI). Inclusion criteria included women within the specified age range without a history of recent spinal surgery. Exclusion criteria included those with systemic illnesses affecting bone health. A total of 500 women were selected through stratified random sampling from both urban and rural areas. The sampling strategy ensured adequate representation of different socioeconomic backgrounds. Results: The prevalence of LBP was found to be 62.4%. Pain management strategies should focus on the large percentage of individuals in the moderate to severe range (VAS 3-8). Patients in the very severe category (VAS 9-10) may require urgent medical attention, advanced pain relief methods, or changes in treatment plans. The majority (77%) of individuals fall into the minimal to moderate disability range (0-40%), suggesting that most people can manage their condition with treatment and lifestyle adjustments. A notable portion (21%) has severe to crippling disability (41-80%), indicating the need for targeted medical and rehabilitative interventions. A small percentage (2%) is bed-bound, signifying extreme impairment requiring specialized care. Conclusion: LBP is highly prevalent among middle-aged women and significantly impacts daily functioning. Preventive strategies, including lifestyle modification and early intervention, are recommended.